美國Chroma公司成立于1991年,位于美國佛洛蒙特州。主要生產熒光濾光片,具有精密光譜控制的能力,能夠各種極窄線寬、光譜邊緣陡直、平面度好的濾光片。Chroma的產品涵蓋TIRF濾光片、干涉濾光片、全內反射濾光片、Raman濾光片、拉曼濾光片、FISH熒光濾光片、應原位雜交濾光片、激發(fā)濾光片、發(fā)射濾光片、二向色鏡/二向色濾光片、陷波濾光片、燃料濾光片、熒光素濾光片、ND濾光片、中性濾光片、中性灰度鏡、截止濾光片、高通濾光片、低通濾光片、帶通濾光片、紫外濾光片、UV濾光片、生物濾光片和醫(yī)學濾光片。
Chroma濾光片配合常見的熒光燃料有DAPI, TRITC, ECPF, CY3, DSred, FITC等等。大體可分為Alexa Fluor熒光染料濾光片、Cy 熒光染料濾光片、核酸相關探針染料濾光片、細胞功能探針濾光片和熒光蛋白濾光片幾大類,Chroma都已經有與之配合的方案。具體型號如下:
Reactive and conjugated probes, Hydroxycoumarin, Aminocoumarin, Methoxycoumarin, Cascade Blue, Pacific Blue, Pacific Orange, Lucifer yellow, NBD, R-Phycoerythrin, (PE), PE-Cy5 conjugates, PE-Cy7 conjugates, Red 613, PerCP, TruRed, FluorX, Fluorescein, BODIPY-FL, TRITC, X-Rhodamine, Lissamine, Rhodamine B, Texas Red, Allophycocyanin, (APC), APC-Cy7 conjugates,
Alexa Fluor 系列熒光染料, Alexa Fluor 350, Alexa Fluor 405, Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 500, Alexa Fluor 514, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 700, Alexa Fluor 750, Alexa Fluor 790,
Cy 系列熒光染料, Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, Cy7,
核酸相關探針染料, Hoechst 33342, DAPI, Hoechst 33258, SYTOX Blue, Chromomycin A3, Mithramycin, YOYO-1, Ethidium Bromide, Acridine Orange, SYTOX Green, TOTO-1, TO-PRO-1, Thiazole Orange, Propidium Iodide, (PI), LDS 751, 7-AAD, SYTOX Orange, TOTO-3, TO-PRO-3, DRAQ5,
細胞功能探針, Indo-1, Fluo-3, DCFH, DHR, SNARF,
熒光蛋白, Y66H, Y66F, EBFP, EBFP2, Azurite, GFPuv, T-Sapphire, Cerulean, mCFP, ECFP, CyPet, Y66W, mKeima-Red, TagCFP, AmCyan1, mTFP1, S65A, Midoriishi Cyan, Wild Type GFP, S65C, TurboGFP, TagGFP, S65L, Emerald, S65T, EGFP, Azami Green, ZsGreen1, TagYFP, EYFP, Topaz, Venus, mCitrine, YPet, TurboYFP, ZsYellow1, Kusabira Orange, mOrange, mKO, TurboRFP, tdTomato, TagRFP, DsRed monomer, DsRed2 (